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low sequence identity
- To: modeller_usage@listsrv.ucsf.edu
- Subject: low sequence identity
- From: Margot Ernst <margot.ernst AT univie.ac.at>
- Date: Thu, 5 Dec 2002 10:56:33 +0100
- Reply-to: margot.ernst AT univie.ac.at
Dear Modeller support,
I am modeling at low sequence identity and I believe to have the following
problem: The homologous scaffold (comprising a core of beta strands) of my
target, after a fisrt round of modeling, displays a (suspiciously) low RMSD
with respect to the template, and a LOT of bad contacts involving the
hydrophobic core. Consequently I think that in my target secondary structure
motifs should be arranged more spaciously. How can I accomplish this in terms
of additional modeling restraints, or chopping up the alignment and modeling
in pieces, or whatever will work?
thanks,
margot