On 4/16/20 8:41 AM, Mariana GONZALEZ MEDINA wrote:
I have observed that the more templates I include in the homology
modeling the least Ca-Ca distance restraints I obtain. I can't
understand this since I would expect to obtain restraints from missing
residues, homology, etc from more templates and therefore more
restraints imposed into my model. What could an explanation to this?
A given CA-CA distance will only have a single restraint on it,
regardless of how many templates you have (although it will become
multimodal). (If Modeller were simply to add restraints for each
template you would end up with a model that is an average of all
templates, which is not physically correct - if one template says a
CA-CA distance is 10A and another says it is 20A, the distance in the
model should be either 10A or 20A, not 15A.)