On 11/27/23 2:50 PM, Yang, Li-Yen via modeller_usage wrote: > I am trying to use the modeler comparative tool in *Chimera* to model > the structure of a protein (in fasta format) by another similar > protein’s structure (in pdb format). > > I first associated this sequence (in fasta) with the corresponding chain > in the pdb, and I used the modeller comparative tool to model the structure. > > However, I’m getting this error: _modeller.SequenceMismatchError: > read_te_291E> Sequence difference between alignment and pdb :
You would need to see the full error message to be sure what the problem is, and that's presumably inside Chimera somewhere. I see you asked a similar question on the Chimera mailing list, so hopefully they can help you. My guess would be that you have at least one non-standard residue in your protein, and Chimera is handling it differently from Modeller.
Ben Webb, Modeller Caretaker